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Biocompatible iron-oxide nanoparticles for the in-situ magnetic separation of bioproducts

Contact Person: Lennart Kleinfeldt

As part of the SynFoBiA project “New Synthesis and Formulation Processes for difficultly soluble active pharmaceutical ingredients and sensitive biopharmaceuticals”, a highly selective and cost-efficient magnetic separation system will be developed. The system works based on functionalized magnetic nanoparticles and is intended for the purification of proteins from biological cultivation media.
In the first step, tailored superparamagnetic nanoparticles are synthesized using the non-aqueous sol-gel method. Subsequently, the particle surface is modified with metal-complex groups. The functionalization allows the chemical bonding of proteins via a 6-Histidine tag. Magnetic separation of the target substance can be achieved by applying an external magnetic field and impurities can be easily washed off. The target protein is captured with high purity by elution with imidazole. The final regeneration of the magnetic particles allows their re-utilization and thereby minimizes the costs of the entire separation process.
This magnetic separation system is already successfully used for the purification of antibody fragments and Protein A from the cell-free supernatant of a culture medium (ex-situ). As a first step towards a continuous separation process, particles are now added to the bioreactor during the biosynthesis of the protein. For this so-called in situ purification of the proteins from the cell culture medium, the functionalized nanoparticles are added to the bacterial suspension during the growth phase of the microorganisms. It turned out that the purification and removal of the product with the functionalized magnetic nanoparticles is possible during biosynthesis (in situ) with similar efficiency and selectivity to the ex-situ process. Hence, the foundation stone for a continuous production and purification of histidine-tagged proteins was laid and could lead to a broad application of magnetic separation techniques in biotechnology.

Team Members

Current Projects
Synthesis, self-assembly and printing of hard-magnetic nanoparticles more

Quanomet, Junior Research Group “Nanofluidics & Particle Manipulation” more

Processing of conjugated nanoparticle hybrid systems for use in biomedical diagnostics more

Fractionation of Nanoparticles by Preparative Gel Electrophoresis subproject of the SPP 2045 more

Advanced lithium-sulfur battery concepts for aviation (aero-LiS-SE) more

Principles of nanoscale matrix additives for fibre composite more

Nanoscale drug carrier systems as therapeutics for targeted tumor therapy more

Small Angle X-ray Scattering as Metrological Instrument – A Correlation Study on the Optical Properties of Semiconductor Nanoparticles as ubiquitous Measure of Particle Size more

Fractionation of Nanoparticles by Preparative Gel Electrophoresis more

Thin nanoparticle films for cathode composites in all solid state batteries more

ElektroBak – Innovative Materials and Concepts for Microbial Electrochemical Systems more

Characteristics of a nanoparticulate coating based on the process chain more

Finished Projects
Synthesis and processing of tailored AZO nanocrystals more

Preparation of multifunctional nanocomposite thin films made of nanosized building blocks more

Process technology of the synthesis of metal oxide nanoparticles by nonaqueous sol gel methods more

Biocompatible iron-oxide nanoparticles for the in-situ magnetic separation of bioproducts more

Small-Molecule Stabilization of ITO Nanoparticles more

AiF – CleanCoat: Development of transparent, photocatalytically active coatings on polymer substrates with long-term stability more

Protein purification with functionalized magnetic nanoparticles more