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Protein purification with functionalized magnetic nanoparticles

Contact Person: Ingke-Christine Masthoff

At the TU Braunschweig, the DFG-funded Collaborative Research Center SFB 578 “From gene to product” investigates the biotechnological preparation of recombinant proteins, e.g. antibodies for therapeutic applications. One of the biggest challenges is the selective purification and isolation of the proteins from the culture medium in which they are produced. A promising technology as an alternative to a chromatographic separation is the magnetic separation, segregating the product directly from the culture medium with the help of functionalized magnetic nanoparticles.
At the Institute of Particle Technology being in charge of the subproject C7 of the SFB, an effective, selective and due to optimum regenerability cost-efficient magnetic separation system for the desired protein is developed. 
For this purpose first of all very small magnetic nanoparticles (10-20nm) are synthesized via the nonaqueous synthesis. This route permits the precise tuning of the particle size and the crystallinity. 

In a second step the particles are functionalized, which means coating the surface with specially designed chemical groups to make a specific binding between desired protein and particle possible, as well as stabilizing them in water.

The used ultra-small nanoparticles possess the advantage of a high specific surface area for interaction with the protein. If an external magnetic field is applied, the particles with the attached proteins are fixed, making a separation of the product and removal of impurities possible. After turning off the magnetic field and successfully separating protein and particle, the nanoparticles can be used for another separation cycle.

Team Members

Current Projects
Synthesis, self-assembly and printing of hard-magnetic nanoparticles more

Quanomet, Junior Research Group “Nanofluidics & Particle Manipulation” more

Processing of conjugated nanoparticle hybrid systems for use in biomedical diagnostics more

Fractionation of Nanoparticles by Preparative Gel Electrophoresis subproject of the SPP 2045 more

Advanced lithium-sulfur battery concepts for aviation (aero-LiS-SE) more

Principles of nanoscale matrix additives for fibre composite more

Nanoscale drug carrier systems as therapeutics for targeted tumor therapy more

Small Angle X-ray Scattering as Metrological Instrument – A Correlation Study on the Optical Properties of Semiconductor Nanoparticles as ubiquitous Measure of Particle Size more

Fractionation of Nanoparticles by Preparative Gel Electrophoresis more

Thin nanoparticle films for cathode composites in all solid state batteries more

ElektroBak – Innovative Materials and Concepts for Microbial Electrochemical Systems more

Characteristics of a nanoparticulate coating based on the process chain more

Finished Projects
Synthesis and processing of tailored AZO nanocrystals more

Preparation of multifunctional nanocomposite thin films made of nanosized building blocks more

Process technology of the synthesis of metal oxide nanoparticles by nonaqueous sol gel methods more

Biocompatible iron-oxide nanoparticles for the in-situ magnetic separation of bioproducts more

Small-Molecule Stabilization of ITO Nanoparticles more

AiF – CleanCoat: Development of transparent, photocatalytically active coatings on polymer substrates with long-term stability more

Protein purification with functionalized magnetic nanoparticles more